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Obligate vernal pool species
 Fairy shrimp are small (about 1 inch) crustaceans which spend their entire lives ( a few weeks) in a vernal pool. Eggs hatch in late winter/early spring and adults may be observed in pools in the spring. Females eventually drop an egg case which remains on the pool bottom after the pool dries. The eggs pass through a cycle of drying and freezing, and then hatch another year when water returns. The presence of fairy shrimp indicates that a water body is a vernal pool.
 Wood frogs are an amphibian species of upland forests. They venture to vernal pools in early spring, lay their eggs, and return to the moist woodland for the remainder of the year. The tadpoles develop in the pool and eventually follow the adults to adjacent uplands. The presence of evidence of breeding by wood frogs (chorusing or mating adults, egg masses or tadpoles) indicates that a pool is a vernal pool.
 The mole salamanders are also upland organisms. They spend most of their lives in burrows on the forest floor. Annually, on certain rainy nights, they migrate to ancestral vernal pools to mate and lay their eggs. They soon return to the upland. The eggs develop in the pool and, by the time the pool dries, the young emerge to begin their life as a terrestrial animal. Evidence that mole salamanders breed in an area make that water body a vernal pool. Breeding evidence would be a breeding congress, spermatophores, egg masses or larvae.
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